URL: http://www.addgene.org/27794
Proper Citation: RRID:Addgene_27794
Insert Name: mVenus C1
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17040988
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pEYFP C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This construct was created by mutagenesis of pEYFP-C1. When compared with EYFP, Venus contains the following mutations: F46L, F64L, M153T, V163A and S175G. This construct also contains A206K mutation to create a monomeric form of the fluorescent protein. This plasmid contains a MCS downstream of mVenus so that a gene of interest can be cloned to create a fusion protein with an N-terminal mVenus tag.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
