URL: http://www.addgene.org/27691
Proper Citation: RRID:Addgene_27691
Insert Name: CALM
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21445324
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cloned from IMAGE clone: 2651109 (MGC:19382). This plasmid may be slow growing in bacteria. For difficult constructs you can use TG1s (they are Endonuclease positive, but good for cloning) and INV F' E.coli strains. The latter give the best results. Also when growing in culture try to switch to 2xTY media, which is richer that LB.
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Source: Addgene
