URL: http://www.addgene.org/26807
Proper Citation: RRID:Addgene_26807
Insert Name: Sterol Regulatory Element Binding Protein 2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15340088
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid expresses the truncated mature SREBP-2 protein (aa 1-482) which is targeted to the nucleus where it activates gene expression and not the full length SREBP-2 inactive precursor protein that is tethered to the endoplasmic reticulum. The plasmid was cloned similarly to the SREBP-1a and 1c constructs that are described in the publication listed below. Please note that the SREBP-2 sequence contains L7V, G76S, K395R and I482L when compared to GenBank entry NP_004590.2. These changes may represent natural variants and have no known functional consequences. This is the exact plasmid used in the associated article listed below.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
