URL: http://www.addgene.org/26745
Proper Citation: RRID:Addgene_26745
Insert Name: iCre expression cassette
Organism: optimized iCre expression cassette; optimized Flpo expression cassette
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20953177
Vector Backbone Description: Backbone Marker:Addgene plasmid 13793; Backbone Size:5200; Vector Backbone:pPGKFlpobpA; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT; Bacterial Resistance:Ampicillin
Comments: The pDIRE plasmid is designed to express the optimized versions of the Cre (iCre) and Flp (Flpo) recombinases and is required for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521 Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906
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Source: Addgene
