URL: http://www.addgene.org/24334
Proper Citation: RRID:Addgene_24334
Insert Name: H2B
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19706417
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation. *H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
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No rating or validation information has been found for hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean.
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Source: Addgene
