URL: http://www.addgene.org/220740
Proper Citation: RRID:Addgene_220740
Insert Name: NIR-FbLAG30
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39242569
Vector Backbone Description: Backbone Marker:Invitrogen/Thermo Fisher Scientific; Backbone Size:5045; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Comments: GenBank: MW627294.1 (part of the insert). To make pNIR-FbLAG30, LAG30 nanobody was amplified from pET21-pelB-LAG-30 (Addgene #172754) and then internally fused to miRFP670nano3 amplified from pcDNA-miRFP670nano3 (Addgene #184664). Construct was inserted to pcDNA3.1 plasmid using KpnI and EcoRI restriction enzymes.
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Source: Addgene
