URL: http://www.addgene.org/219536
Proper Citation: RRID:Addgene_219536
Insert Name: Cre recombinase
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: 1. A restriction-based cloning method is highly recommended for any changes/manipulation of this plasmid. Amplification of this plasmid OR restriction-free cloning enzymes may lead to undesired recombination. 2. The inserts "EF1A-Cre-MS2-RBGpA" and "UbC-GFP-pA" are placed in opposite orientations to ensure efficient lentivirus packaging. Further design consideration is based on https://www.intechopen.com/chapters/16787. Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.
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No rating or validation information has been found for pLenti-Cre-MS2_UbC-GFP (Cre_GFP).
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Source: Addgene