URL: http://www.addgene.org/192838
Proper Citation: RRID:Addgene_192838
Insert Name: mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
Organism: Synthetic
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: When using ∆G rabies virus of the CVS-N2c strain, rather than the SAD B19 strain, AAV1 made using this plasmid should be used instead of 100799-AAV1, as part of the two-helper-virus combination described (for SAD B19) in PMID 28847002, 31408693, and 32116642. The virus should be diluted to the same concentration as described for 100799-AAV1 and otherwise used as recommended in those publications.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
