URL: http://www.addgene.org/15458
Proper Citation: RRID:Addgene_15458
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:17512993
Vector Backbone Description: Backbone Size:8019; Vector Backbone:N/A; Vector Types:S. aureus-E. coli shuttle vector; Bacterial Resistance:Spectinomycin
Comments: pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB. In addition, pLL39 has a tandem terminator at one side of the multiple cloning sites to prevent read through of the cloned gene from an external promoter once integrated into the chromosome.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
