URL: http://www.addgene.org/12543
Proper Citation: RRID:Addgene_12543
Insert Name: NRas 61K
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8668210
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The original N-Ras was cloned as a full-length cDNA (containing 5' and 3' UTRs) Bam-Bam into pCGN, and was therefore transfered via Bam cuts into other vectors, including pBABE, and orientation checked. So, a 1.2 kb band is expected. There should be about 100 bp of 5'UTR, 527 bp N-Ras, and the rest 3'UTR
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Source: Addgene
