URL: http://www.addgene.org/12282
Proper Citation: RRID:Addgene_12282
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12370261
Vector Backbone Description: Backbone Size:7166; Vector Backbone:modified pMIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Please acknowledge Dr. Yosef Refaeli (National Jewish Medical and Research Center) when using this plasmid in future publications. pMIGw is a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold. IMPORTANT: This plasmid grows extremely slowly! Addgene recommends that when prepping DNA, you let your culture grow 18-20 hours (or more). Overnight will probably not be sufficient for a good prep.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
