URL: http://www.addgene.org/12178
Proper Citation: RRID:Addgene_12178
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15105502
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: The firefly luciferase vector was modified from pGL3 Control Vector (Promega), such that a short sequence containing multiple cloning sites (5'-AGCTCTATACGCGTCTCAAGCTTACTGCTAGC GT-3') was inserted into the XbaI site immediately downstream from the stop codon. 3'UTR segments of target genes can be inserted into this vector to test for their effects on mRNA stability.
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Source: Addgene
