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PerCP/Cyanine5.5 anti-mouse CD69 antibody

RRID:AB_940497

Antibody ID

AB_940497

Target Antigen

CD69 See NCBI gene mouse

Proper Citation

(BioLegend Cat# 104521, RRID:AB_940497)

Clonality

monoclonal antibody

Reference

PMID:27855783

Comments

Applications: FC

Clone ID

Clone H1.2F3

Host Organism

armenian hamster

Vendor

BioLegend Go To Vendor

Cat Num

104521

Publications that use this research resource

Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy.

  • Qi S
  • Elife
  • 2016 Nov 18

Literature context:


Abstract:

The combined-immunotherapy of adoptive cell therapy (ACT) and cyclophosphamide (CTX) is one of the most efficient treatments for melanoma patients. However, no synergistic effects of CTX and ACT on the spatio-temporal dynamics of immunocytes in vivo have been described. Here, we visualized key cell events in immunotherapy-elicited immunoreactions in a multicolor-coded tumor microenvironment, and then established an optimal strategy of metronomic combined-immunotherapy to enhance anti-tumor efficacy. Intravital imaging data indicated that regulatory T cells formed an 'immunosuppressive ring' around a solid tumor. The CTX-ACT combined-treatment elicited synergistic immunoreactions in tumor areas, which included relieving the immune suppression, triggering the transient activation of endogenous tumor-infiltrating immunocytes, increasing the accumulation of adoptive cytotoxic T lymphocytes, and accelerating the infiltration of dendritic cells. These insights into the spatio-temporal dynamics of immunocytes are beneficial for optimizing immunotherapy and provide new approaches for elucidating the mechanisms underlying the involvement of immunocytes in cancer immunotherapy.

Funding information:
  • NIH HHS - P51 OD011133(United States)