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APC/Cyanine7 anti-mouse TCR ? chain antibody

RRID:AB_893624

Antibody ID

AB_893624

Target Antigen

TCR-b chain See NCBI gene mouse

Proper Citation

(BioLegend Cat# 109220, RRID:AB_893624)

Clonality

monoclonal antibody

Comments

Applications: FC

Clone ID

Clone H57-597

Host Organism

armenian hamster

Vendor

BioLegend Go To Vendor

Cat Num

109220

Publications that use this research resource

Transcription Factor IRF8 Orchestrates the Adaptive Natural Killer Cell Response.

  • Adams NM
  • Immunity
  • 2018 Jun 19

Literature context:


Abstract:

Natural killer (NK) cells are innate lymphocytes that display features of adaptive immunity during viral infection. Biallelic mutations in IRF8 have been reported to cause familial NK cell deficiency and susceptibility to severe viral infection in humans; however, the precise role of this transcription factor in regulating NK cell function remains unknown. Here, we show that cell-intrinsic IRF8 was required for NK-cell-mediated protection against mouse cytomegalovirus infection. During viral exposure, NK cells upregulated IRF8 through interleukin-12 (IL-12) signaling and the transcription factor STAT4, which promoted epigenetic remodeling of the Irf8 locus. Moreover, IRF8 facilitated the proliferative burst of virus-specific NK cells by promoting expression of cell-cycle genes and directly controlling Zbtb32, a master regulator of virus-driven NK cell proliferation. These findings identify the function and cell-type-specific regulation of IRF8 in NK-cell-mediated antiviral immunity and provide a mechanistic understanding of viral susceptibility in patients with IRF8 mutations.

Funding information:
  • NCI NIH HHS - P01CA104106(United States)

CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

  • Hirata Y
  • Cell Stem Cell
  • 2018 Mar 1

Literature context:


Abstract:

A crucial player in immune regulation, FoxP3+ regulatory T cells (Tregs) are drawing attention for their heterogeneity and noncanonical functions. Here, we describe a Treg subpopulation that controls hematopoietic stem cell (HSC) quiescence and engraftment. These Tregs highly expressed an HSC marker, CD150, and localized within the HSC niche in the bone marrow (BM). Specific reduction of BM Tregs achieved by conditional deletion of CXCR4 in Tregs increased HSC numbers in the BM. Adenosine generated via the CD39 cell surface ectoenzyme on niche Tregs protected HSCs from oxidative stress and maintained HSC quiescence. In transplantation settings, niche Tregs prevented allogeneic (allo-) HSC rejection through adenosine and facilitated allo-HSC engraftment. Furthermore, transfer of niche Tregs promoted allo-HSC engraftment to a much greater extent than transfer of other Tregs. These results identify a unique niche-associated Treg subset and adenosine as regulators of HSC quiescence, abundance, and engraftment, further highlighting their therapeutic utility.

Funding information:
  • NIDDK NIH HHS - R01 DK051665(United States)