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ABCG1 antibody [EP1366Y]

RRID:AB_867471

Antibody ID

AB_867471

Target Antigen

ABCG1 antibody [EP1366Y] bovine, human, mouse, rat, cow

Proper Citation

(Abcam Cat# ab52617, RRID:AB_867471)

Clonality

monoclonal antibody

Comments

validation status unknown, seller recommendations provided in 2012: Immunohistochemistry; Immunocytochemistry; Immunohistochemistry - fixed; Western Blot; ICC, IHC-P, WB

Host Organism

rabbit

Vendor

Abcam

Cat Num

ab52617

Publications that use this research resource

Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1β Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis.

  • Tumurkhuu G
  • Cell Metab.
  • 2018 Jun 18

Literature context:


Abstract:

Pathogen burden accelerates atherosclerosis, but the mechanisms remain unresolved. Activation of the NLRP3 inflammasome is linked to atherogenesis. Here we investigated whether Chlamydia pneumoniae (C.pn) infection engages NLRP3 in promoting atherosclerosis. C.pn potentiated hyperlipidemia-induced inflammasome activity in cultured macrophages and in foam cells in atherosclerotic lesions of Ldlr-/- mice. C.pn-induced acceleration of atherosclerosis was significantly dependent on NLRP3 and caspase-1. We discovered that C.pn-induced extracellular IL-1β triggers a negative feedback loop to inhibit GPR109a and ABCA1 expression and cholesterol efflux, leading to accumulation of intracellular cholesterol and foam cell formation. Gpr109a and Abca1 were both upregulated in plaque lesions in Nlrp3-/- mice in both hyperlipidemic and C.pn infection models. Mature IL-1β and cholesterol may compete for access to the ABCA1 transporter to be exported from macrophages. C.pn exploits this metabolic-immune crosstalk, which can be modulated by NLRP3 inhibitors to alleviate atherosclerosis.

Funding information:
  • NCI NIH HHS - R01 CA095614(United States)
  • NHLBI NIH HHS - R01 HL066436()
  • NHLBI NIH HHS - R01 HL111483()
  • NIAID NIH HHS - R21 AI105845()