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TRAF6 antibody [EP591Y]

RRID:AB_778572

Antibody ID

AB_778572

Target Antigen

TRAF6 antibody [EP591Y] human, mouse, rat, mouse, rat, human

Proper Citation

(Abcam Cat# ab33915, RRID:AB_778572)

Clonality

monoclonal antibody

Comments

validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Western Blot; Immunocytochemistry; Immunohistochemistry - fixed; Immunohistochemistry; Immunoprecipitation; Flow Cyt, IHC-P, IP, WB

Host Organism

rabbit

Vendor

Abcam

Cat Num

ab33915

Publications that use this research resource

YOD1/TRAF6 association balances p62-dependent IL-1 signaling to NF-κB.

  • Schimmack G
  • Elife
  • 2017 Feb 28

Literature context: ), TRAF6 (RRID:AB_778572 (WB)) (all


Abstract:

The ubiquitin ligase TRAF6 is a key regulator of canonical IκB kinase (IKK)/NF-κB signaling in response to interleukin-1 (IL-1) stimulation. Here, we identified the deubiquitinating enzyme YOD1 (OTUD2) as a novel interactor of TRAF6 in human cells. YOD1 binds to the C-terminal TRAF homology domain of TRAF6 that also serves as the interaction surface for the adaptor p62/Sequestosome-1, which is required for IL-1 signaling to NF-κB. We show that YOD1 competes with p62 for TRAF6 association and abolishes the sequestration of TRAF6 to cytosolic p62 aggregates by a non-catalytic mechanism. YOD1 associates with TRAF6 in unstimulated cells but is released upon IL-1β stimulation, thereby facilitating TRAF6 auto-ubiquitination as well as NEMO/IKKγ substrate ubiquitination. Further, IL-1 triggered IKK/NF-κB signaling and induction of target genes is decreased by YOD1 overexpression and augmented after YOD1 depletion. Hence, our data define that YOD1 antagonizes TRAF6/p62-dependent IL-1 signaling to NF-κB.