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Anti-Mouse CD19 Biotin 500 ug antibody

RRID:AB_657658

Antibody ID

AB_657658

Target Antigen

Mouse CD19 500 ug mouse

Proper Citation

(Thermo Fisher Scientific Cat# 13-0193-85, RRID:AB_657658)

Clonality

monoclonal antibody

Comments

Applications: Flow (0.25 µg/test)

Clone ID

Clone eBio1D3 (1D3)

Host Organism

rat

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

13-0193-85

Publications that use this research resource

Transformation of Accessible Chromatin and 3D Nucleome Underlies Lineage Commitment of Early T Cells.

  • Hu G
  • Immunity
  • 2018 Feb 20

Literature context:


Abstract:

How chromatin reorganization coordinates differentiation and lineage commitment from hematopoietic stem and progenitor cells (HSPCs) to mature immune cells has not been well understood. Here, we carried out an integrative analysis of chromatin accessibility, topologically associating domains, AB compartments, and gene expression from HSPCs to CD4+CD8+ T cells. We found that abrupt genome-wide changes at all three levels of chromatin organization occur during the transition from double-negative stage 2 (DN2) to DN3, accompanying the T lineage commitment. The transcription factor BCL11B, a critical regulator of T cell commitment, is associated with increased chromatin interaction, and Bcl11b deletion compromised chromatin interaction at its target genes. We propose that these large-scale and concerted changes in chromatin organization present an energy barrier to prevent the cell from reversing its fate to earlier stages or redirecting to alternatives and thus lock the cell fate into the T lineages.

Funding information:
  • Intramural NIH HHS - Z01 HL005801-05()
  • NIAID NIH HHS - R01 AI083514()
  • NIGMS NIH HHS - R01GM073943(United States)