URL: http://antibodyregistry.org/AB_572266
Proper Citation: (ImmunoStar Cat# 20064, RRID:AB_572266)
Target Antigen: Substance P
Host Organism: rabbit
Clonality: polyclonal
Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.; Gene Symbol: Tacr1
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- Human colon Whole Mount technique staining in Myenteric plexus in Soma shows strong immunostaining. Human colon Whole Mount technique staining in Myenteric plexus in Fibers shows strong immunostaining. Data provided by Brookes lab. - Brookes et al. (2022) via SPARC https://sparc.science/resources/7Mlidjv3RIVrQ11hpBC8PK
No alerts have been found for Substance P Antibody.
Source: Antibody Registry
