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Anti-Mouse CD62L (L-Selectin) PE 50 ug antibody

RRID:AB_465720

Antibody ID

AB_465720

Target Antigen

Mouse CD62L (L-Selectin) PE 50 ug mouse

Proper Citation

(Thermo Fisher Scientific Cat# 12-0621-81, RRID:AB_465720)

Clonality

monoclonal antibody

Comments

Applications: Flow (0.125 µg/test)

Clone ID

Clone MEL-14

Host Organism

rat

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

12-0621-81

Publications that use this research resource

Genomic Characterization of Murine Monocytes Reveals C/EBPβ Transcription Factor Dependence of Ly6C- Cells.

  • Mildner A
  • Immunity
  • 2017 May 16

Literature context:


Abstract:

Monocytes are circulating, short-lived mononuclear phagocytes, which in mice and man comprise two main subpopulations. Murine Ly6C+ monocytes display developmental plasticity and are recruited to complement tissue-resident macrophages and dendritic cells on demand. Murine vascular Ly6C- monocytes patrol the endothelium, act as scavengers, and support vessel wall repair. Here we characterized population and single cell transcriptomes, as well as enhancer and promoter landscapes of the murine monocyte compartment. Single cell RNA-seq and transplantation experiments confirmed homeostatic default differentiation of Ly6C+ into Ly6C- monocytes. The main two subsets were homogeneous, but linked by a more heterogeneous differentiation intermediate. We show that monocyte differentiation occurred through de novo enhancer establishment and activation of pre-established (poised) enhancers. Generation of Ly6C- monocytes involved induction of the transcription factor C/EBPβ and C/EBPβ-deficient mice lacked Ly6C- monocytes. Mechanistically, C/EBPβ bound the Nr4a1 promoter and controlled expression of this established monocyte survival factor.

Funding information:
  • Intramural NIH HHS - ZIA BC010876-03(United States)