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Mouse Anti-Histone H3, phospho (Ser10) Monoclonal Antibody, Unconjugated, Clone mAbcam 14955

RRID:AB_443110

Antibody ID

AB_443110

Target Antigen

Histone H3 (phospho S10) bovine, canine, chicken/avian, donkey, drosophila, feline, guinea pig, hamster, human, mouse, other, porcine, rabbit, rat, sheep, simian, xenopus, yeast, reacts with human, mouse, african green monkey, fruit fly (drosophila melanogaster), indian muntjac and xenopus laevispredicted to react with all mammals, arabidopsis thaliana, aspergillus nidulans, caenorhabditis elegans, chicken, chlamydomonas reinhardtii, neurospora crassa, saccharomyces cerevisiae, schizosaccharomyces pombe, tobacco and zebrafish due to sequence homology

Vendor

Abcam

Cat Num

ab14955

Proper Citation

(Abcam Cat# ab14955, RRID:AB_443110)

Clonality

monoclonal antibody

Clone ID

Clone mAbcam 14955

Host Organism

mouse

Comments

validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot; Chromatin IP, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-P, IP, Western Blot

Publications that use this research resource

The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding.

  • Liu J
  • Cell Stem Cell
  • 2017 Nov 2

Literature context: Cat# ab14955;RRID:AB_443110 Rabbit polyclonal anti-HOPX Sig


Abstract:

Human brain evolution is associated with expansion and folding of the neocortex. Increased diversity in neural progenitor (NP) populations (such as basally located radial glia [RG], which reside in an enlarged outer subventricular zone [OSVZ]) likely contributes to this evolutionary expansion, although their characteristics and relative contributions are only partially understood. Through single-cell transcriptional profiling of sorted human NP subpopulations, we identified the primate-specific TMEM14B gene as a marker of basal RG. Expression of TMEM14B in embryonic NPs induces cortical thickening and gyrification in postnatal mice. This is accompanied by SVZ expansion, the appearance of outer RG-like cells, and the proliferation of multiple NP subsets, with proportional increases in all cortical layers and normal lamination. TMEM14B drives NP proliferation by increasing the phosphorylation and nuclear translocation of IQGAP1, which in turn promotes G1/S cell cycle transitions. These data show that a single primate-specific gene can drive neurodevelopmental changes that contribute to brain evolution.