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Ras-GAP antibody

RRID:AB_397455

Antibody ID

AB_397455

Target Antigen

Ras-GAP chicken, dog, frog, human, mouse, rat, human, xenopus/amphibian, chicken/bird, canine, mouse, rat

Vendor

BD Biosciences

Cat Num

610040

Proper Citation

(BD Biosciences Cat# 610040, RRID:AB_397455)

Reference

PMID:27759562

Clonality

monoclonal antibody

Host Organism

mouse

Comments

validation status unknown, vendor suggested use: IgG1; IgG1 Immunofluorescence, Immunohistochemistry, Western Blot, Immunoprecipitation; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blot

Publications that use this research resource

TP53 exon-6 truncating mutations produce separation of function isoforms with pro-tumorigenic functions.

  • Shirole NH
  • Elife
  • 2016 Oct 19

TP53 truncating mutations are common in human tumors and are thought to give rise to p53-null alleles. Here, we show that TP53 exon-6 truncating mutations occur at higher than expected frequencies and produce proteins that lack canonical p53 tumor suppressor activities but promote cancer cell proliferation, survival, and metastasis. Functionally and molecularly, these p53 mutants resemble the naturally occurring alternative p53 splice variant, p53-psi. Accordingly, these mutants can localize to the mitochondria where they promote tumor phenotypes by binding and activating the mitochondria inner pore permeability regulator, Cyclophilin D (CypD). Together, our studies reveal that TP53 exon-6 truncating mutations, contrary to current beliefs, act beyond p53 loss to promote tumorigenesis, and could inform the development of strategies to target cancers driven by these prevalent mutations.