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Rabbit Anti-Mouse IgM , Unconjugated antibody

RRID:AB_390193

Antibody ID

AB_390193

Target Antigen

Mouse IgM mouse

Proper Citation

(Millipore Cat# 12-488, RRID:AB_390193)

Clonality

unknown

Comments

seller recommendations: Immunoprecipitation; Western Blot; Immunoprecipitation, Western Blotting

Host Organism

rabbit

Vendor

Millipore

Cat Num

12-488

Publications that use this research resource

PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes.

  • Endoh M
  • Elife
  • 2017 Mar 17

Literature context:


Abstract:

The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust de-repression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.