X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

APC anti-mouse IFN-? antibody

RRID:AB_315403

Antibody ID

AB_315403

Target Antigen

IFN-g See NCBI gene mouse

Proper Citation

(BioLegend Cat# 505809, RRID:AB_315403)

Clonality

monoclonal antibody

Comments

Applications: ICFC

Clone ID

Clone XMG1.2

Host Organism

rat

Vendor

BioLegend Go To Vendor

Cat Num

505809

Publications that use this research resource

Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut Microbiota.

  • Cignarella F
  • Cell Metab.
  • 2018 Jun 5

Literature context:


Abstract:

Multiple sclerosis (MS) is more common in western countries with diet being a potential contributing factor. Here we show that intermittent fasting (IF) ameliorated clinical course and pathology of the MS model, experimental autoimmune encephalomyelitis (EAE). IF led to increased gut bacteria richness, enrichment of the Lactobacillaceae, Bacteroidaceae, and Prevotellaceae families and enhanced antioxidative microbial metabolic pathways. IF altered T cells in the gut with a reduction of IL-17 producing T cells and an increase in regulatory T cells. Fecal microbiome transplantation from mice on IF ameliorated EAE in immunized recipient mice on a normal diet, suggesting that IF effects are at least partially mediated by the gut flora. In a pilot clinical trial in MS patients, intermittent energy restriction altered blood adipokines and the gut flora resembling protective changes observed in mice. In conclusion, IF has potent immunomodulatory effects that are at least partially mediated by the gut microbiome.

Funding information:
  • NCI NIH HHS - R01 CA126942(United States)

Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy.

  • Qi S
  • Elife
  • 2016 Nov 18

Literature context:


Abstract:

The combined-immunotherapy of adoptive cell therapy (ACT) and cyclophosphamide (CTX) is one of the most efficient treatments for melanoma patients. However, no synergistic effects of CTX and ACT on the spatio-temporal dynamics of immunocytes in vivo have been described. Here, we visualized key cell events in immunotherapy-elicited immunoreactions in a multicolor-coded tumor microenvironment, and then established an optimal strategy of metronomic combined-immunotherapy to enhance anti-tumor efficacy. Intravital imaging data indicated that regulatory T cells formed an 'immunosuppressive ring' around a solid tumor. The CTX-ACT combined-treatment elicited synergistic immunoreactions in tumor areas, which included relieving the immune suppression, triggering the transient activation of endogenous tumor-infiltrating immunocytes, increasing the accumulation of adoptive cytotoxic T lymphocytes, and accelerating the infiltration of dendritic cells. These insights into the spatio-temporal dynamics of immunocytes are beneficial for optimizing immunotherapy and provide new approaches for elucidating the mechanisms underlying the involvement of immunocytes in cancer immunotherapy.

Funding information:
  • NIH HHS - P51 OD011133(United States)