X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

FITC anti-mouse CD8a antibody

RRID:AB_312744

Antibody ID

AB_312744

Target Antigen

CD8a See NCBI gene mouse

Proper Citation

(BioLegend Cat# 100705, RRID:AB_312744)

Clonality

monoclonal antibody

Comments

Applications: FC

Clone ID

Clone 53-6.7

Host Organism

rat

Vendor

BioLegend Go To Vendor

Cat Num

100705

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 iCreTbx21 fl/fl mice.

  • Cuff AO
  • Wellcome Open Res
  • 2017 Aug 18

Literature context:


Abstract:

Mouse liver contains both Eomes-dependent conventional natural killer (cNK) cells and Tbet-dependent liver-resident type I innate lymphoid cells (ILC1). In order to better understand the role of ILC1, we attempted to generate mice that would lack liver ILC1, while retaining cNK, by conditional deletion of Tbet in NKp46+ cells. Here we report that the Ncr1 iCreTbx21 fl/fl mouse has a roughly equivalent reduction in both the cNK and ILC1 compartments of the liver, limiting its utility for investigating the relative contributions of these two cell types in disease models. We also describe the phenotype of these mice with respect to NK cells, ILC1 and NKp46 + ILC3 in the spleen and small intestine lamina propria.

Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy.

  • Qi S
  • Elife
  • 2016 Nov 18

Literature context:


Abstract:

The combined-immunotherapy of adoptive cell therapy (ACT) and cyclophosphamide (CTX) is one of the most efficient treatments for melanoma patients. However, no synergistic effects of CTX and ACT on the spatio-temporal dynamics of immunocytes in vivo have been described. Here, we visualized key cell events in immunotherapy-elicited immunoreactions in a multicolor-coded tumor microenvironment, and then established an optimal strategy of metronomic combined-immunotherapy to enhance anti-tumor efficacy. Intravital imaging data indicated that regulatory T cells formed an 'immunosuppressive ring' around a solid tumor. The CTX-ACT combined-treatment elicited synergistic immunoreactions in tumor areas, which included relieving the immune suppression, triggering the transient activation of endogenous tumor-infiltrating immunocytes, increasing the accumulation of adoptive cytotoxic T lymphocytes, and accelerating the infiltration of dendritic cells. These insights into the spatio-temporal dynamics of immunocytes are beneficial for optimizing immunotherapy and provide new approaches for elucidating the mechanisms underlying the involvement of immunocytes in cancer immunotherapy.

Funding information:
  • NIH HHS - P51 OD011133(United States)