X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Anti-Mps1, NT, clone 3-472-1 antibody

RRID:AB_309903

Antibody ID

AB_309903

Target Antigen

Mps1, NT human

Proper Citation

(Millipore Cat# 05-682, RRID:AB_309903)

Clonality

monoclonal antibody

Reference

PMID:28463114

Comments

seller recommendations: Western Blot; Western Blotting

Clone ID

Clone 3-472-1

Host Organism

mouse

Vendor

Millipore

Cat Num

05-682

Publications that use this research resource

Protein Phosphatase 1 inactivates Mps1 to ensure efficient Spindle Assembly Checkpoint silencing.

  • Moura M
  • Elife
  • 2017 May 2

Literature context:


Abstract:

Faithfull genome partitioning during cell division relies on the Spindle Assembly Checkpoint (SAC), a conserved signaling pathway that delays anaphase onset until all chromosomes are attached to spindle microtubules. Mps1 kinase is an upstream SAC regulator that promotes the assembly of an anaphase inhibitor through a sequential multi-target phosphorylation cascade. Thus, the SAC is highly responsive to Mps1, whose activity peaks in early mitosis as a result of its T-loop autophosphorylation. However, the mechanism controlling Mps1 inactivation once kinetochores attach to microtubules and the SAC is satisfied remains unknown. Here we show in vitro and in Drosophila that Protein Phosphatase 1 (PP1) inactivates Mps1 by dephosphorylating its T-loop. PP1-mediated dephosphorylation of Mps1 occurs at kinetochores and in the cytosol, and inactivation of both pools of Mps1 during metaphase is essential to ensure prompt and efficient SAC silencing. Overall, our findings uncover a mechanism of SAC inactivation required for timely mitotic exit.