X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Rabbit Anti-VSV-G tag Polyclonal Antibody, Unconjugated

RRID:AB_302646

Antibody ID

AB_302646

Target Antigen

VSV-G tag other

Proper Citation

(Abcam Cat# ab1874, RRID:AB_302646)

Clonality

polyclonal antibody

Reference

PMID:27549340

Comments

validation status unknown, seller recommendations provided in 2012: Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blot; Immunocytochemistry, Immunohistochemistry-Fr, Immunoprecipitation, Western Blot

Host Organism

rabbit

Vendor

Abcam

Cat Num

ab1874

Publications that use this research resource

Sleep deprivation causes memory deficits by negatively impacting neuronal connectivity in hippocampal area CA1.

  • Havekes R
  • Elife
  • 2016 Aug 23

Literature context:


Abstract:

Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity have been proposed as a substrate of memory storage. Here, we examine the impact of brief periods of sleep deprivation on dendritic structure. In mice, we find that five hours of sleep deprivation decreases dendritic spine numbers selectively in hippocampal area CA1 and increased activity of the filamentous actin severing protein cofilin. Recovery sleep normalizes these structural alterations. Suppression of cofilin function prevents spine loss, deficits in hippocampal synaptic plasticity, and impairments in long-term memory caused by sleep deprivation. The elevated cofilin activity is caused by cAMP-degrading phosphodiesterase-4A5 (PDE4A5), which hampers cAMP-PKA-LIMK signaling. Attenuating PDE4A5 function prevents changes in cAMP-PKA-LIMK-cofilin signaling and cognitive deficits associated with sleep deprivation. Our work demonstrates the necessity of an intact cAMP-PDE4-PKA-LIMK-cofilin activation-signaling pathway for sleep deprivation-induced memory disruption and reduction in hippocampal spine density.