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Rat Anti-Laminin 2 alpha Monoclonal Antibody, Unconjugated, Clone 4H8-2

RRID:AB_298180

Antibody ID

AB_298180

Target Antigen

Laminin 2 alpha human, mouse, reacts with human and mousenot yet tested in other species

Proper Citation

(Abcam Cat# ab11576, RRID:AB_298180)

Clonality

monoclonal antibody

Comments

validation status unknown, seller recommendations provided in 2012: ELISA; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blot; ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Fr, Immunoprecipitation, Western Blot

Clone ID

Clone 4H8-2

Host Organism

rat

Vendor

Abcam

Cat Num

ab11576

Publications that use this research resource

Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates.

  • Mead AF
  • Elife
  • 2017 Nov 22

Literature context: alpha antibody (4H8-2, ab11576 (RRID:AB_298180), Abcam, MA, USA), diluted 1:50


Abstract:

Superfast muscles (SFMs) are extremely fast synchronous muscles capable of contraction rates up to 250 Hz, enabling precise motor execution at the millisecond time scale. SFM phenotypes have been discovered in most major vertebrate lineages, but it remains unknown whether all SFMs share excitation-contraction coupling pathway adaptations for speed, and if SFMs arose once, or from independent evolutionary events. Here, we demonstrate that to achieve rapid actomyosin crossbridge kinetics bat and songbird SFM express myosin heavy chain genes that are evolutionarily and ontologically distinct. Furthermore, we show that all known SFMs share multiple functional adaptations that minimize excitation-contraction coupling transduction times. Our results suggest that SFM evolved independently in sound-producing organs in ray-finned fish, birds, and mammals, and that SFM phenotypes operate at a maximum operational speed set by fundamental constraints in synchronous muscle. Consequentially, these constraints set a fundamental limit to the maximum speed of fine motor control.

Funding information:
  • NHLBI NIH HHS - R01 HL087103(United States)