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anti-phospho-Thr737-Sch9 antibody

RRID:AB_2722566

Antibody ID

AB_2722566

Target Antigen

Proper Citation

(GeneCust Cat# A008748, RRID:AB_2722566)

Clonality

polyclonal antibody

Reference

PMID:29570051

Host Organism

rabbit

Vendor

GeneCust

Cat Num

A008748

Publications that use this research resource

The yeast H+-ATPase Pma1 promotes Rag/Gtr-dependent TORC1 activation in response to H+-coupled nutrient uptake.

  • Saliba E
  • Elife
  • 2018 Mar 23

Literature context:


Abstract:

The yeast Target of Rapamycin Complex 1 (TORC1) plays a central role in controlling growth. How amino acids and other nutrients stimulate its activity via the Rag/Gtr GTPases remains poorly understood. We here report that the signal triggering Rag/Gtr-dependent TORC1 activation upon amino-acid uptake is the coupled H+ influx catalyzed by amino-acid/H+ symporters. H+-dependent uptake of other nutrients, ionophore-mediated H+ diffusion, and inhibition of the vacuolar V-ATPase also activate TORC1. As the increase in cytosolic H+ elicited by these processes stimulates the compensating H+-export activity of the plasma membrane H+-ATPase (Pma1), we have examined whether this major ATP-consuming enzyme might be involved in TORC1 control. We find that when the endogenous Pma1 is replaced with a plant H+-ATPase, H+ influx or increase fails to activate TORC1. Our results show that H+ influx coupled to nutrient uptake stimulates TORC1 activity and that Pma1 is a key actor in this mechanism.

Funding information:
  • Fonds De La Recherche Scientifique - FNRS - 22396499()
  • Fonds De La Recherche Scientifique - FNRS - 3.4.592.08.F()
  • Fonds De La Recherche Scientifique - FNRS - 30274494()
  • Fonds National de La Recherche Scientifique - 22396499()
  • Fonds National de La Recherche Scientifique - 3.4.592.08.F()
  • Fonds National de La Recherche Scientifique - 30274494()
  • Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture - 21074048()
  • Intramural NIH HHS - (United States)