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recombinant human monoclonal antibody to HIV-1 gp120 2G12

RRID:AB_2661842

Antibody ID

AB_2661842

Target Antigen

HIV-1 gp120 Env human

Proper Citation

(Polymun Cat# AB002, RRID:AB_2661842)

Clonality

monoclonal antibody

Reference

PMID:28574338

Comments

RUO

Clone ID

2G12

Host Organism

recombinant cho

Vendor

Polymun

Cat Num

AB002

Publications that use this research resource

Synchronized HIV assembly by tunable PIP2 changes reveals PIP2 requirement for stable Gag anchoring.

  • M├╝cksch F
  • Elife
  • 2017 Jun 2

Literature context: Cat#AB002 RRID:AB_2661842) / Fab Fra


Abstract:

HIV-1 assembles at the plasma membrane (PM) of infected cells. PM association of the main structural protein Gag depends on its myristoylated MA domain and PM PI(4,5)P2. Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 completely prevents Gag PM targeting and assembly site formation. Unexpectedly, PI(4,5)P2 depletion also caused loss of pre-assembled Gag lattices from the PM. Subsequent restoration of PM PI(4,5)P2 reinduced assembly site formation even in the absence of new protein synthesis, indicating that the dissociated Gag molecules remained assembly competent. These results reveal an important role of PI(4,5)P2 for HIV-1 morphogenesis beyond Gag recruitment to the PM and suggest a dynamic equilibrium of Gag-lipid interactions. Furthermore, they establish an experimental system that permits synchronized induction of HIV-1 assembly leading to induced production of infectious virions by targeted modulation of Gag PM targeting.