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Green fluorescent protein (GFP) antibody - DSHB; University of Iowa


Antibody ID


Target Antigen

Green fluorescent protein (GFP) aequorea victoria all species as fusion proteins

Proper Citation

(DSHB Cat# DSHB-GFP-1D2, RRID:AB_2617419)


monoclonal antibody




Application(s): Immunofluorescence,Western Blot; Date Deposited: 05/17/2016

Host Organism



DSHB Go To Vendor

Cat Num


Publications that use this research resource

The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology.

  • Fu Y
  • Elife
  • 2018 Jan 29

Literature context:


We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, Trichoplusia ni, assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families reveal T. ni-specific gene expansions that may explain its widespread distribution and rapid adaptation to insecticides. Transcriptome and small RNA data from thorax, ovary, testis, and the germline-derived Hi5 cell line show distinct expression profiles for 295 microRNA- and >393 piRNA-producing loci, as well as 39 genes encoding small RNA pathway proteins. Nearly all of the W chromosome is devoted to piRNA production, and T. ni siRNAs are not 2ยด-O-methylated. To enable use of Hi5 cells as a model system, we have established genome editing and single-cell cloning protocols. The T. ni genome provides insights into pest control and allows Hi5 cells to become a new tool for studying small RNAs ex vivo.

Funding information:
  • NIMH NIH HHS - R21-MH099812(United States)