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Green fluorescent protein (GFP) antibody - DSHB; University of Iowa

RRID:AB_2617419

Antibody ID

AB_2617419

Target Antigen

Green fluorescent protein (GFP) aequorea victoria all species as fusion proteins

Proper Citation

(DSHB Cat# DSHB-GFP-1D2, RRID:AB_2617419)

Clonality

monoclonal antibody

Reference

PMID:29376823

Comments

Application(s): Immunofluorescence,Western Blot; Date Deposited: 05/17/2016

Host Organism

mouse

Vendor

DSHB Go To Vendor

Cat Num

DSHB-GFP-1D2

Publications that use this research resource

The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology.

  • Fu Y
  • Elife
  • 2018 Jan 29

Literature context:


Abstract:

We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, Trichoplusia ni, assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families reveal T. ni-specific gene expansions that may explain its widespread distribution and rapid adaptation to insecticides. Transcriptome and small RNA data from thorax, ovary, testis, and the germline-derived Hi5 cell line show distinct expression profiles for 295 microRNA- and >393 piRNA-producing loci, as well as 39 genes encoding small RNA pathway proteins. Nearly all of the W chromosome is devoted to piRNA production, and T. ni siRNAs are not 2ยด-O-methylated. To enable use of Hi5 cells as a model system, we have established genome editing and single-cell cloning protocols. The T. ni genome provides insights into pest control and allows Hi5 cells to become a new tool for studying small RNAs ex vivo.

Funding information:
  • NIMH NIH HHS - R21-MH099812(United States)