URL: http://antibodyregistry.org/AB_260754
Proper Citation: (Sigma-Aldrich Cat# N2280, RRID:AB_260754)
Target Antigen: Nitric Oxide Synthase
Host Organism: mouse
Clonality: monoclonal
Comments: Applications: western blot analysis, immunocytochemistry, double immunofluorescence, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), dot blot immunoassay, immunohistochemical staining
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No rating or validation information has been found for Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse.
Possibly Non-Specific
Extracted Antibody Information: "1B), which were also present in the nNOS-deficient tissue, suggesting that they were non-specific. The third antibody (Sigma Aldrich; N2280; AB_260754) exhibited clear immunolabelling of cell bodies in the wild-type tissue, which was completely absent in the nNOS-deficient tissue (Fig. 1C)."
Extracted Specificity Statement: "The second antibody (Cell Signaling; 4234; AB_10694499) displayed some sparse immunoreactivity “spots” that could be mistaken for cell bodies within the VTA and SNc (Fig. 1B), which were also present in the nNOS-deficient tissue, suggesting that they were non-specific. The third antibody (Sigma Aldrich; N2280; AB_260754) exhibited clear immunolabelling of cell bodies in the wild-type tissue, which was completely absent in the nNOS-deficient tissue (Fig."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:30456293
Possibly Non-Specific
Extracted Antibody Information: "also exhibited somewhat non-specific immunolabelling, that was only partially absent in tissue from nNOS-deficient mice. C, Anti-nNOS (Sigma Aldrich; N2280; AB_260754)"
Extracted Specificity Statement: "The second antibody (Cell Signaling; 4234; AB_10694499) displayed some sparse immunoreactivity “spots” that could be mistaken for cell bodies within the VTA and SNc (Fig. 1B), which were also present in the nNOS-deficient tissue, suggesting that they were non-specific. The third antibody (Sigma Aldrich; N2280; AB_260754) exhibited clear immunolabelling of cell bodies in the wild-type tissue, which was completely absent in the nNOS-deficient tissue (Fig."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:30456293
Source: Antibody Registry
