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Mouse IL-6 ELISA Ready-SET-Go!¨ 2 x 96 tests Kit

RRID:AB_2574986

Antibody ID

AB_2574986

Target Antigen

IL-6 mouse

Proper Citation

(Thermo Fisher Scientific Cat# 88-7064-22, RRID:AB_2574986)

Clonality

unknown

Comments

Discontinued; Original Manufacturer of this product eBioscience, now part of Thermo Fisher; tested applications: ELISA (ELISA)

Vendor

Thermo Fisher Scientific

Cat Num

88-7064-22 also 88-7064

Publications that use this research resource

Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets.

  • Macdougall CE
  • Cell Metab.
  • 2018 Mar 6

Literature context:


Abstract:

Visceral adipose tissue (VAT) has multiple roles in orchestrating whole-body energy homeostasis. In addition, VAT is now considered an immune site harboring an array of innate and adaptive immune cells with a direct role in immune surveillance and host defense. We report that conventional dendritic cells (cDCs) in VAT acquire a tolerogenic phenotype through upregulation of pathways involved in adipocyte differentiation. While activation of the Wnt/β-catenin pathway in cDC1 DCs induces IL-10 production, upregulation of the PPARγ pathway in cDC2 DCs directly suppresses their activation. Combined, they promote an anti-inflammatory milieu in vivo delaying the onset of obesity-induced chronic inflammation and insulin resistance. Under long-term over-nutrition, changes in adipocyte biology curtail β-catenin and PPARγ activation, contributing to VAT inflammation.

Funding information:
  • PHS HHS - R01-54044(United States)

RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity.

  • McKelvey AC
  • Elife
  • 2016 Nov 2

Literature context:


Abstract:

Toll-like receptor 2 (TLR2) is a pattern recognition receptor that recognizes many types of PAMPs that originate from gram-positive bacteria. Here we describe a novel mechanism regulating TLR2 protein expression and subsequent cytokine release through the ubiquitination and degradation of the receptor in response to ligand stimulation. We show a new mechanism in which an uncharacterized RING finger E3 ligase, PPP1R11, directly ubiquitinates TLR2 both in vitro and in vivo, which leads to TLR2 degradation and disruption of the signaling cascade. Lentiviral gene transfer or knockdown of PPP1R11 in mouse lungs significantly affects lung inflammation and the clearance of Staphylococcus aureus. There is a negative correlation between PPP1R11 and TLR2 levels in white blood cell samples isolated from patients with Staphylococcus aureus infections. These results suggest that PPP1R11 plays an important role in regulating innate immunity and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2.

Funding information:
  • NIMH NIH HHS - R56MH104593(United States)