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PV (parvalbumin) antibody

RRID:AB_2571615

Antibody ID

AB_2571615

Target Antigen

mouse parvalbumin (NM013645) mouse

Proper Citation

(Frontier Institute Cat# PV-GP, RRID:AB_2571615)

Clonality

polyclonal antibody

Host Organism

guinea pig

Vendor

Frontier Institute Go To Vendor

Cat Num

PV-GP

Publications that use this research resource

Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain.

  • Nishiyama J
  • Neuron
  • 2017 Nov 15

Literature context:


Abstract:

Precise genome editing via homology-directed repair (HDR) in targeted cells, particularly in vivo, provides an invaluable tool for biomedical research. However, HDR has been considered to be largely restricted to dividing cells, making it challenging to apply the technique in postmitotic neurons. Here we show that precise genome editing via HDR is possible in mature postmitotic neurons as well as mitotic cells in mice brain by combining CRISPR-Cas9-mediated DNA cleavage and the efficient delivery of donor template with adeno-associated virus (AAV). Using this strategy, we achieved efficient tagging of endogenous proteins in primary and organotypic cultures in vitro and developing, adult, aged, and pathological brains in vivo. Thus, AAV- and CRISPR-Cas9-mediated HDR will be broadly useful for precise genome editing in basic and translational neuroscience.

Funding information:
  • NIMH NIH HHS - R01 MH080047()
  • NINDS NIH HHS - DP1 NS096787()