X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

barren antibody

RRID:AB_2567044

Antibody ID

AB_2567044

Target Antigen

barr d melanogaster

Vendor

Bhat MA; Cell. 1996 Go To Vendor

Cat Num

barr

Proper Citation

(Bhat MA; Cell. 1996 Cat# barr, RRID:AB_2567044)

Clonality

polyclonal antibody

Comments

Lab generated antibody, submitted by FlyBase FBgn0014127

Publications that use this research resource

Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation.

  • Piskadlo E
  • Elife
  • 2017 May 6

Literature context: ., 1996), RRID:AB_2567044).


Abstract:

Mitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of de novo Topoisomerase II (TopoII)-dependent re-entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re-intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.