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Brilliant Violet 711™ anti-human CD3 antibody


Antibody ID


Target Antigen

CD3 See NCBI gene human, cross-reactivity: chimpanzee

Proper Citation

(BioLegend Cat# 300463, RRID:AB_2566035)


monoclonal antibody




Applications: FC

Clone ID

Clone UCHT1

Host Organism



BioLegend Go To Vendor

Cat Num


Publications that use this research resource

Simultaneous enumeration of cancer and immune cell types from bulk tumor gene expression data.

  • Racle J
  • Elife
  • 2017 Nov 13

Literature context:


Immune cells infiltrating tumors can have important impact on tumor progression and response to therapy. We present an efficient algorithm to simultaneously estimate the fraction of cancer and immune cell types from bulk tumor gene expression data. Our method integrates novel gene expression profiles from each major non-malignant cell type found in tumors, renormalization based on cell-type-specific mRNA content, and the ability to consider uncharacterized and possibly highly variable cell types. Feasibility is demonstrated by validation with flow cytometry, immunohistochemistry and single-cell RNA-Seq analyses of human melanoma and colorectal tumor specimens. Altogether, our work not only improves accuracy but also broadens the scope of absolute cell fraction predictions from tumor gene expression data, and provides a unique novel experimental benchmark for immunogenomics analyses in cancer research (http://epic.gfellerlab.org).