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Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550

RRID:AB_2556607

Antibody ID

AB_2556607

Target Antigen

Rat IgG (H+L) Cross-Adsorbed rat

Proper Citation

(Thermo Fisher Scientific Cat# SA5-10027, RRID:AB_2556607)

Clonality

polyclonal antibody

Comments

Applications: Flow (1:50-1:200), ICC (2 µg/mL), IF (2 µg/mL), IHC (1:50 - 500), IP (Assay Dependent), WB (1:5,000-1:20,000)

Host Organism

donkey

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

SA5-10027

Publications that use this research resource

Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis.

  • Winter C
  • Cell Metab.
  • 2018 Jul 3

Literature context: l) Thermo Fisher Cat#SA5-10027; RRID:AB_2556607 Goat anti-armenian hamster IgG


Abstract:

Onset of cardiovascular complications as a consequence of atherosclerosis exhibits a circadian incidence with a peak in the morning hours. Although development of atherosclerosis extends for long periods of time through arterial leukocyte recruitment, we hypothesized that discrete diurnal invasion of the arterial wall could sustain atherogenic growth. Here, we show that myeloid cell recruitment to atherosclerotic lesions oscillates with a peak during the transition from the activity to the resting phase. This diurnal phenotype is regulated by rhythmic release of myeloid cell-derived CCL2, and blockade of its signaling abolished oscillatory leukocyte adhesion. In contrast, we show that myeloid cell adhesion to microvascular beds peaks during the early activity phase. Consequently, timed pharmacological CCR2 neutralization during the activity phase caused inhibition of atherosclerosis without disturbing microvascular recruitment. These findings demonstrate that chronic inflammation of large vessels feeds on rhythmic myeloid cell recruitment, and lay the foundation for chrono-pharmacology-based therapy.

Funding information:
  • NIEHS NIH HHS - P42 ES005948(United States)