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DYKDDDDK Tag Monoclonal Antibody (L5)

RRID:AB_2536846

Antibody ID

AB_2536846

Target Antigen

DYKDDDDK Tag tag

Proper Citation

(Thermo Fisher Scientific Cat# MA1-142, RRID:AB_2536846)

Clonality

monoclonal antibody

Comments

Applications: WB (1 µg/mL), ELISA (100 ng/mL)

Clone ID

Clone L5

Host Organism

rat

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

MA1-142

Publications that use this research resource

Npas4 Is a Critical Regulator of Learning-Induced Plasticity at Mossy Fiber-CA3 Synapses during Contextual Memory Formation.

  • Weng FJ
  • Neuron
  • 2018 Mar 7

Literature context: ag tag Thermo Fisher Scientific RRID:AB_2536846 Mouse monoclonal anti-HA tag Co


Abstract:

Synaptic connections between hippocampal mossy fibers (MFs) and CA3 pyramidal neurons are essential for contextual memory encoding, but the molecular mechanisms regulating MF-CA3 synapses during memory formation and the exact nature of this regulation are poorly understood. Here we report that the activity-dependent transcription factor Npas4 selectively regulates the structure and strength of MF-CA3 synapses by restricting the number of their functional synaptic contacts without affecting the other synaptic inputs onto CA3 pyramidal neurons. Using an activity-dependent reporter, we identified CA3 pyramidal cells that were activated by contextual learning and found that MF inputs on these cells were selectively strengthened. Deletion of Npas4 prevented both contextual memory formation and this learning-induced synaptic modification. We further show that Npas4 regulates MF-CA3 synapses by controlling the expression of the polo-like kinase Plk2. Thus, Npas4 is a critical regulator of experience-dependent, structural, and functional plasticity at MF-CA3 synapses during contextual memory formation.

Funding information:
  • FIC NIH HHS - 1R03TW008208(United States)
  • NIDA NIH HHS - R01 DA017392()
  • NIDCD NIH HHS - R01 DC014701()
  • NIMH NIH HHS - R01 MH081935()
  • NIMH NIH HHS - R01 MH091220()
  • NINDS NIH HHS - R21 NS088412()