X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

GFP Tag Polyclonal Antibody, HRP

RRID:AB_2534022

Antibody ID

AB_2534022

Target Antigen

GFP Tag tag

Proper Citation

(Thermo Fisher Scientific Cat# A10260, RRID:AB_2534022)

Clonality

polyclonal antibody

Comments

Applications: WB (1:200-1:2000), IHC (1:200-1:2000)

Host Organism

rabbit

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

A10260

Publications that use this research resource

Vg1-Nodal heterodimers are the endogenous inducers of mesendoderm.

  • Montague TG
  • Elife
  • 2017 Nov 15

Literature context:


Abstract:

Nodal is considered the key inducer of mesendoderm in vertebrate embryos and embryonic stem cells. Other TGF-beta-related signals, such as Vg1/Dvr1/Gdf3, have also been implicated in this process but their roles have been unclear or controversial. Here we report that zebrafish embryos without maternally provided vg1 fail to form endoderm and head and trunk mesoderm, and closely resemble nodal loss-of-function mutants. Although Nodal is processed and secreted without Vg1, it requires Vg1 for its endogenous activity. Conversely, Vg1 is unprocessed and resides in the endoplasmic reticulum without Nodal, and is only secreted, processed and active in the presence of Nodal. Co-expression of Nodal and Vg1 results in heterodimer formation and mesendoderm induction. Thus, mesendoderm induction relies on the combination of two TGF-beta-related signals: maternal and ubiquitous Vg1, and zygotic and localized Nodal. Modeling reveals that the pool of maternal Vg1 enables rapid signaling at low concentrations of zygotic Nodal.