X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Alexa Fluor 680-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) antibody

RRID:AB_2340868

Antibody ID

AB_2340868

Target Antigen

Mouse IgG (H+L)

Proper Citation

(Jackson ImmunoResearch Labs Cat# 715-625-150, RRID:AB_2340868)

Clonality

polyclonal antibody

Reference

PMID:28825401

Comments

Originating manufacturer of this product

Vendor

Jackson ImmunoResearch Labs Go To Vendor

Cat Num

715-625-150

Publications that use this research resource

A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade.

  • Purice MD
  • Elife
  • 2017 Aug 21

Literature context:


Abstract:

Neural injury triggers swift responses from glia, including glial migration and phagocytic clearance of damaged neurons. The transcriptional programs governing these complex innate glial immune responses are still unclear. Here, we describe a novel injury assay in adult Drosophila that elicits widespread glial responses in the ventral nerve cord (VNC). We profiled injury-induced changes in VNC gene expression by RNA sequencing (RNA-seq) and found that responsive genes fall into diverse signaling classes. One factor, matrix metalloproteinase-1 (MMP-1), is induced in Drosophila ensheathing glia responding to severed axons. Interestingly, glial induction of MMP-1 requires the highly conserved engulfment receptor Draper, as well as AP-1 and STAT92E. In MMP-1 depleted flies, glia do not properly infiltrate neuropil regions after axotomy and, as a consequence, fail to clear degenerating axonal debris. This work identifies Draper-dependent activation of MMP-1 as a novel cascade required for proper glial clearance of severed axons.