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Anti-Rabbit IgG (H+L), made in goat antibody

RRID:AB_2336194

Antibody ID

AB_2336194

Target Antigen

IgG rabbit

Proper Citation

(Vector Laboratories Cat# AP-1000, RRID:AB_2336194)

Clonality

unknown

Reference

PMID:28504929

Host Organism

goat

Vendor

Vector Laboratories

Cat Num

AP-1000

Publications that use this research resource

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

  • Nillegoda NB
  • Elife
  • 2017 May 15

Literature context:


Abstract:

Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 for disaggregation. We assessed the prevalence and evolutionary conservation of J-protein complexation and cooperation in disaggregation. We find the emergence of a eukaryote-specific signature for interclass complexation of canonical J-proteins. Consistently, complexes exist in yeast and human cells, but not in bacteria, and correlate with cooperative action in disaggregation in vitro. Signature alterations exclude some J-proteins from networking, which ensures correct J-protein pairing, functional network integrity and J-protein specialization. This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes.