URL: http://antibodyregistry.org/AB_221539
Proper Citation: Molecular Probes Cat# A-11132, RRID:AB_221539
Target Antigen: beta-galactosidase
Host Organism: rabbit
Clonality: polyclonal
Comments: Discontinued; This product offered by Molecular Probes (Invitrogen), now part of Thermo Fisher: Genomics and Molecular Biology; Reporter Gene Detection; Beta-Galactosidase (LacZ) Substrates and Assay Kits
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No rating or validation information has been found for Rabbit Anti-beta-galactosidase Polyclonal Antibody, Unconjugated.
Discontinued
Discontinued at Molecular Probes
Issue
This antibody recognizes the bacterial β-galactosidase protein, but not the mammalian β-galactosidase protein. It has been used by authors to detect the mammalian protein, do not do this it will not work.
"While endogenous mammalian β-galactosidase protein is used as a marker for senescent cells, while the E. coli β-galactosidase protein is a component of the versatile LacZ reporter system used for genetics experiments. While these enzymes have the same name, catalyse the same reaction, and share a useful substrate in X-Gal, they are otherwise related to separate lines of scientific inquiry." - Sholto David (for better science blog)
Source: Antibody Registry
