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Mouse TROP-2 Affinity Purified Polyclonal Ab antibody

RRID:AB_2205662

Antibody ID

AB_2205662

Target Antigen

Mouse TROP-2 Affinity Purified Ab mouse

Proper Citation

(R and D Systems Cat# AF1122, RRID:AB_2205662)

Clonality

polyclonal antibody

Comments

vendor recommendations: IgG Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot; Flow Cytometry; Western Blot; Immunohistochemistry; Immunocytochemistry

Host Organism

goat

Vendor

R and D Systems

Cat Num

AF1122

Publications that use this research resource

YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.

  • Yui S
  • Cell Stem Cell
  • 2018 Jan 4

Literature context: tems Cat #: AF1122, RRID:AB_2205662 Alexa Flour 488 Donkey anti Rat


Abstract:

Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element.

Funding information:
  • NCI NIH HHS - R01 CA084197-11(United States)