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Mouse KLF4 Affinity Purified Polyclonal Ab antibody

RRID:AB_2130245

Antibody ID

AB_2130245

Target Antigen

Mouse KLF4 Affinity Purified Ab mouse

Proper Citation

(R and D Systems Cat# AF3158, RRID:AB_2130245)

Clonality

polyclonal antibody

Comments

vendor recommendations: IgG Immunohistochemistry; Western Blot; Immunocytochemistry; Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Western Blot

Host Organism

goat

Vendor

R and D Systems

Cat Num

AF3158

Publications that use this research resource

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

  • Morgani SM
  • Elife
  • 2018 Feb 7

Literature context: F4 R and D Systems Cat# AF3158, RRID:AB_2130245 1:200


Abstract:

During gastrulation epiblast cells exit pluripotency as they specify and spatially arrange the three germ layers of the embryo. Similarly, human pluripotent stem cells (PSCs) undergo spatially organized fate specification on micropatterned surfaces. Since in vivo validation is not possible for the human, we developed a mouse PSC micropattern system and, with direct comparisons to mouse embryos, reveal the robust specification of distinct regional identities. BMP, WNT, ACTIVIN and FGF directed mouse epiblast-like cells to undergo an epithelial-to-mesenchymal transition and radially pattern posterior mesoderm fates. Conversely, WNT, ACTIVIN and FGF patterned anterior identities, including definitive endoderm. By contrast, epiblast stem cells, a developmentally advanced state, only specified anterior identities, but without patterning. The mouse micropattern system offers a robust scalable method to generate regionalized cell types present in vivo, resolve how signals promote distinct identities and generate patterns, and compare mechanisms operating in vivo and in vitro and across species.

Funding information:
  • Cancer Research UK - 06-914/915(United Kingdom)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development - R01HD080699()
  • National Cancer Institute - P30CA008748()
  • National Institute of Diabetes and Digestive and Kidney Diseases - R01DK084391()
  • National Science Foundation - PHY1502151()
  • NYSTEM - C029568()