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GATA-3 (D-16) antibody

RRID:AB_2108588

Antibody ID

AB_2108588

Target Antigen

GATA-3 (D-16) mouse, rat, rabbit, mouse, rat, human, canine, human

Proper Citation

(Santa Cruz Biotechnology Cat# sc-22206, RRID:AB_2108588)

Clonality

polyclonal antibody

Comments

Discontinued: 2016; validation status unknown check with seller; recommendations: Immunofluorescence; Immunohistochemistry; WB, IP, IF, IHC(P), ELISA; Immunocytochemistry; Immunoprecipitation; Western Blot; ELISA

Host Organism

goat

Vendor

Santa Cruz Biotechnology

Cat Num

sc-22206

Publications that use this research resource

Molecular conservation of marsupial and eutherian placentation and lactation.

  • Guernsey MW
  • Elife
  • 2017 Sep 12

Literature context:


Abstract:

Eutherians are often mistakenly termed 'placental mammals', but marsupials also have a placenta to mediate early embryonic development. Lactation is necessary for both infant and fetal development in eutherians and marsupials, although marsupials have a far more complex milk repertoire that facilitates morphogenesis of developmentally immature young. In this study, we demonstrate that the anatomically simple tammar placenta expresses a dynamic molecular program that is reminiscent of eutherian placentation, including both fetal and maternal signals. Further, we provide evidence that genes facilitating fetal development and nutrient transport display convergent co-option by placental and mammary gland cell types to optimize offspring success.

Glucocorticoid Receptor:MegaTrans Switching Mediates the Repression of an ERα-Regulated Transcriptional Program.

  • Yang F
  • Mol. Cell
  • 2017 May 4

Literature context:


Abstract:

The molecular mechanisms underlying the opposing functions of glucocorticoid receptors (GRs) and estrogen receptor α (ERα) in breast cancer development remain poorly understood. Here we report that, in breast cancer cells, liganded GR represses a large ERα-activated transcriptional program by binding, in trans, to ERα-occupied enhancers. This abolishes effective activation of these enhancers and their cognate target genes, and it leads to the inhibition of ERα-dependent binding of components of the MegaTrans complex. Consistent with the effects of SUMOylation on other classes of nuclear receptors, dexamethasone (Dex)-induced trans-repression of the estrogen E2 program appears to depend on GR SUMOylation, which leads to stable trans-recruitment of the GR-N-CoR/SMRT-HDAC3 corepressor complex on these enhancers. Together, these results uncover a mechanism by which competitive recruitment of DNA-binding nuclear receptors/transcription factors in trans to hot spot enhancers serves as an effective biological strategy for trans-repression, with clear implications for breast cancer and other diseases.

Funding information:
  • Howard Hughes Medical Institute - R01 CA213371()
  • NCI NIH HHS - R01 DK018477()
  • NIDDK NIH HHS - R01 DK039949()