URL: http://antibodyregistry.org/AB_2092506
Proper Citation: Proteintech Cat# 17721-1-AP, RRID:AB_2092506
Target Antigen: DHX9
Host Organism: rabbit
Clonality: polyclonal
Comments:
Originating manufacturer of this product.
Applications: WB, RNA pull-down, IHC, IF, ELISA
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No rating or validation information has been found for DHX9 antibody.
Possibly Non-Specific
Extracted Antibody Information: "The following day, the membrane was incubated for 1 hour at room temperature with the primary antibody solution in 1:20 western blocking reagent diluted in TBST. Antibodies used included rabbit polyclonal anti-TRIM25 (1:1000, ab167154, clone EPR7315, Abcam), rabbit polyclonal anti-DHX9 (1:1000, 17721-1-AP, RRID – AB_2092506,"
Extracted Specificity Statement: "Lanes 3, 6, and 9 show pre-let-7a-1 pull-down. Note that T7 antibody highlights an unspecific band visible in lanes 3 and 8. f Western blot analyses against T7 and DHX9 of pre-let-7a-1 pull-downs with HeLa TRIM25 KO cell extracts overexpressing T7-TRIM25, T7-TRIM25ΔRBD, or T7-TRIM25ΔCC. Lanes 1, 4, and 7 represent 4% (40 μg) of the loading controls (input)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:29117863
Possibly Non-Specific
Extracted Antibody Information: "The following day, the membrane was incubated for 1 hour at room temperature with the primary antibody solution in 1:20 western blocking reagent diluted in TBST. Antibodies used included rabbit polyclonal anti-TRIM25 (1:1000, ab167154, clone EPR7315, Abcam), rabbit polyclonal anti-DHX9 (1:1000, 17721-1-AP, RRID – AB_2092506,"
Extracted Specificity Statement: "Lanes 3, 6, and 9 show pre-let-7a-1 pull-down. Note that the T7 antibody highlights an unspecific band visible in lanes 3 and 8. d Western blot analyses against T7 and DHX9 of pre-let-7a-1 pull-downs with HeLa cell extracts overexpressing T7-TRIM25/5, T7-TRIM25/21, or T7-TRIM25/27. Lanes 1, 4, and 7 represent 4% (40 μg) of the loading controls (input)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:29117863
Source: Antibody Registry
