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AQP5 (G-19) antibody

RRID:AB_2059877

Antibody ID

AB_2059877

Target Antigen

AQP5 (G-19) mouse, rat, human, mouse, rat, human

Proper Citation

(Santa Cruz Biotechnology Cat# sc-9890, RRID:AB_2059877)

Clonality

polyclonal antibody

Comments

Discontinued: 2016; validation status unknown check with seller; recommendations: WB, IP, IF, ELISA; Immunoprecipitation; Western Blot; Immunofluorescence; ELISA

Host Organism

goat

Vendor

Santa Cruz Biotechnology

Cat Num

sc-9890

Publications that use this research resource

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

  • Nikolić MZ
  • Elife
  • 2017 Jun 30

Literature context:


Abstract:

The embryonic mouse lung is a widely used substitute for human lung development. For example, attempts to differentiate human pluripotent stem cells to lung epithelium rely on passing through progenitor states that have only been described in mouse. The tip epithelium of the branching mouse lung is a multipotent progenitor pool that self-renews and produces differentiating descendants. We hypothesized that the human distal tip epithelium is an analogous progenitor population and tested this by examining morphology, gene expression and in vitro self-renewal and differentiation capacity of human tips. These experiments confirm that human and mouse tips are analogous and identify signalling pathways that are sufficient for long-term self-renewal of human tips as differentiation-competent organoids. Moreover, we identify mouse-human differences, including markers that define progenitor states and signalling requirements for long-term self-renewal. Our organoid system provides a genetically-tractable tool that will allow these human-specific features of lung development to be investigated.