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Rabbit Anti-beta-Tubulin Monoclonal Antibody, HRP Conjugated, Clone 9F3

RRID:AB_1950376

Antibody ID

AB_1950376

Target Antigen

beta-Tubulin bovine, chicken/avian, fish, human, mouse, rat, simian, human, mouse, rat, monkey, bovine, zebrafish, chicken

Proper Citation

(Cell Signaling Technology Cat# 5346, RRID:AB_1950376)

Clonality

monoclonal antibody

Comments

Applications: W. Consolidation on 11/2018: AB_10694412, AB_1950376.

Clone ID

Clone 9F3

Host Organism

rabbit

Vendor

Cell Signaling Technology

Cat Num

5346

Publications that use this research resource

Acetyl-CoA Carboxylase 1-Dependent Protein Acetylation Controls Breast Cancer Metastasis and Recurrence.

  • Rios Garcia M
  • Cell Metab.
  • 2017 Dec 5

Literature context:


Abstract:

Breast tumor recurrence and metastasis represent the main causes of cancer-related death in women, and treatments are still lacking. Here, we define the lipogenic enzyme acetyl-CoA carboxylase (ACC) 1 as a key player in breast cancer metastasis. ACC1 phosphorylation was increased in invading cells both in murine and human breast cancer, serving as a point of convergence for leptin and transforming growth factor (TGF) β signaling. ACC1 phosphorylation was mediated by TGFβ-activated kinase (TAK) 1, and ACC1 inhibition was indispensable for the elevation of cellular acetyl-CoA, the subsequent increase in Smad2 transcription factor acetylation and activation, and ultimately epithelial-mesenchymal transition and metastasis induction. ACC1 deficiency worsened tumor recurrence upon primary tumor resection in mice, and ACC1 phosphorylation levels correlated with metastatic potential in breast and lung cancer patients. Given the demonstrated effectiveness of anti-leptin receptor antibody treatment in halting ACC1-dependent tumor invasiveness, our work defines a "metabolocentric" approach in metastatic breast cancer therapy.

Funding information:
  • NIDDK NIH HHS - P30 DK065988(United States)