X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Anti-Human CD317 (BST2/PDCA-1) Functional Grade Purified 100 ug antibody

RRID:AB_1518775

Antibody ID

AB_1518775

Target Antigen

Human CD317 (BST2/PDCA-1) Functional Grade Purified 100 ug human

Proper Citation

(Thermo Fisher Scientific Cat# 16-3179-82, RRID:AB_1518775)

Clonality

monoclonal antibody

Comments

Applications: FN (Assay-Dependent), Flow (0.125 µg/test), Neu (Assay-Dependent)

Clone ID

Clone 26F8

Host Organism

mouse

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

16-3179-82

Publications that use this research resource

Synchronized HIV assembly by tunable PIP2 changes reveals PIP2 requirement for stable Gag anchoring.

  • Mücksch F
  • Elife
  • 2017 Jun 2

Literature context:


Abstract:

HIV-1 assembles at the plasma membrane (PM) of infected cells. PM association of the main structural protein Gag depends on its myristoylated MA domain and PM PI(4,5)P2. Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 completely prevents Gag PM targeting and assembly site formation. Unexpectedly, PI(4,5)P2 depletion also caused loss of pre-assembled Gag lattices from the PM. Subsequent restoration of PM PI(4,5)P2 reinduced assembly site formation even in the absence of new protein synthesis, indicating that the dissociated Gag molecules remained assembly competent. These results reveal an important role of PI(4,5)P2 for HIV-1 morphogenesis beyond Gag recruitment to the PM and suggest a dynamic equilibrium of Gag-lipid interactions. Furthermore, they establish an experimental system that permits synchronized induction of HIV-1 assembly leading to induced production of infectious virions by targeted modulation of Gag PM targeting.