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Anti-Human CD3 (TCR complex) Monoclonal Antibody, Allophycocyanin Conjugated, Clone UCHT1

RRID:AB_130788

Antibody ID

AB_130788

Target Antigen

Human CD3 (TCR complex) Allophycocyanin Clone UCHT1 human

Proper Citation

(Beckman Coulter Cat# IM2467, RRID:AB_130788)

Clonality

monoclonal antibody

Reference

PMID:29130882

Comments

manufacturer recommendations: IgG1 Flow Cytometry; Flow Cytometry

Vendor

Beckman Coulter

Cat Num

IM2467

Publications that use this research resource

Simultaneous enumeration of cancer and immune cell types from bulk tumor gene expression data.

  • Racle J
  • Elife
  • 2017 Nov 13

Literature context:


Abstract:

Immune cells infiltrating tumors can have important impact on tumor progression and response to therapy. We present an efficient algorithm to simultaneously estimate the fraction of cancer and immune cell types from bulk tumor gene expression data. Our method integrates novel gene expression profiles from each major non-malignant cell type found in tumors, renormalization based on cell-type-specific mRNA content, and the ability to consider uncharacterized and possibly highly variable cell types. Feasibility is demonstrated by validation with flow cytometry, immunohistochemistry and single-cell RNA-Seq analyses of human melanoma and colorectal tumor specimens. Altogether, our work not only improves accuracy but also broadens the scope of absolute cell fraction predictions from tumor gene expression data, and provides a unique novel experimental benchmark for immunogenomics analyses in cancer research (http://epic.gfellerlab.org).