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FOXA2 antibody [EPR4466]

RRID:AB_11157157

Antibody ID

AB_11157157

Target Antigen

FOXA2 antibody [EPR4466] human, rat, mouse, human, mouse, rat

Proper Citation

(Abcam Cat# ab108422, RRID:AB_11157157)

Clonality

monoclonal antibody

Comments

validation status unknown, seller recommendations provided in 2012: ICC, IHC-P, WB; Immunohistochemistry - fixed; Immunohistochemistry; Western Blot; Immunocytochemistry

Host Organism

rabbit

Vendor

Abcam

Cat Num

ab108422

Publications that use this research resource

Generation of integration-free induced pluripotent stem cell line (NJMUi001-A) from a phenylketonuria patient.

  • Xu T
  • Stem Cell Res
  • 2018 Mar 19

Literature context: OXA2 1:200 Abcam Cat# ab108422, RRID:AB_11157157 Secondary antibodies Goat anti-


Abstract:

PKU is a prevalent type of inherited metabolic disease, caused by the defective phenylalanine metabolism. In most PKU cases, mutations in the PAH gene could be found. Dysfunction of this hepatic enzyme will lead to diverse clinical symptoms due to a failure in converting phenylalanine into tyrosine. Here, we report an integration-free human induced pluripotent stem cell line (NJMUi001-A) generated from peripheral blood mononuclear cells of a PKU patient by using Sendai virus. This iPS cell line has characteristics of pluripotent stem cells and can be used as a useful tool for the investigation of this inherited metabolic disease.

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

  • Morgani SM
  • Elife
  • 2018 Feb 7

Literature context: anti-FOXA2 Abcam Cat# ab108422, RRID:AB_11157157 1:500


Abstract:

During gastrulation epiblast cells exit pluripotency as they specify and spatially arrange the three germ layers of the embryo. Similarly, human pluripotent stem cells (PSCs) undergo spatially organized fate specification on micropatterned surfaces. Since in vivo validation is not possible for the human, we developed a mouse PSC micropattern system and, with direct comparisons to mouse embryos, reveal the robust specification of distinct regional identities. BMP, WNT, ACTIVIN and FGF directed mouse epiblast-like cells to undergo an epithelial-to-mesenchymal transition and radially pattern posterior mesoderm fates. Conversely, WNT, ACTIVIN and FGF patterned anterior identities, including definitive endoderm. By contrast, epiblast stem cells, a developmentally advanced state, only specified anterior identities, but without patterning. The mouse micropattern system offers a robust scalable method to generate regionalized cell types present in vivo, resolve how signals promote distinct identities and generate patterns, and compare mechanisms operating in vivo and in vitro and across species.

Funding information:
  • Cancer Research UK - 06-914/915(United Kingdom)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development - R01HD080699()
  • National Cancer Institute - P30CA008748()
  • National Institute of Diabetes and Digestive and Kidney Diseases - R01DK084391()
  • National Science Foundation - PHY1502151()
  • NYSTEM - C029568()